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VCRB :: Contents of Volume 56/2002 :: Detailed information


PLANT RECOVERY FROM BUD MICRO-EXPLANTS OF SQUASH (CUCURBITA MAXIMA DUCH.)

Maciej ŁADYŻYŃSKI, Wojciech BURZA, Stefan MALEPSZY
Warsaw Agriculture UniversityNowoursynowska 166, 02-766 Warszawa, Poland


Summary

A method was developed for rapid in vitro propagation 
of plants using micro-explants of two winter squash
(Cucurbita maxima Duch.) genotypes: cultivar "Amazonka"
and highly inbred line 62. Axillary and apical buds were
taken from in vitro-growing seedlings, precultured in liquid
medium containing BAP for 10 days, then cut into tiny pieces
and cultured on agar medium, supplemented with cytokinin
and auxin, on which shoots were produced. Explants of line 62
produced more shoots than explants of "Amazonka"
(3.7 and 2.2, respectively). Induction of shoot formation,
probably from already present meristems and by direct,
adventitious organogenesis, required cytokinin BAP
as the sole growth regulator, but the addition of IAA increased
efficiency of this process. Such an effect generally was not
observed for NAA. The best result for 62 line i.e. 7.5 shoots
per bud was obtained on media with 1.0 mgdm-3 BAP and
0.05 mg dm-3 IAA. For "Amazonka" mean values never
exceeded 4 shoots. No new phenotypes were observed
among plants of the R0 generation cultivated in the greenhouse
and the R1 generation grown in the field, suggesting that this
procedure does not induce somaclonal variation.


keywords:in vitro propagation, somaclonal variation, winter squash

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