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VCRB :: Contents of Volume 53/2000 :: Detailed information


METHODS OF DISINFECTING CUCUMBER SEEDS THAT ORIGINATE FROM PLANTS INFECTED BY CUCUMBER GREEN MOTTLE MOSAIC TOBAMOVIRUS (CGMMV)

Władysław MACIAS
Research Institute of Vegetable Crops Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland


Summary

Experiments on disinfection of cucumber seeds that originate from plants infected by cucumber green mottle mosaic tobamovirus were conducted over 1996-1998. The usefulness of following treatments was investigated: 1% HCl, 0.6% CH 3COOH, 0.3-0.5% NaOCl, 1% Ca(OCl)2, 1% sodium toluene-p-sulphonchloramide, 5% CaO, 1% NaOH, 10% Na3PO4, an experimental seed treatment, and seed thermotherapy in 70oC during 3 days. The heat treatment was efficient with fresh seeds only, while 2 years old seeds almost totally lost their ability to germinate. Optimum concentrations for disinfection purposes were as follows: HCl 0.5-1.0%, NaOCl 0.3-0.5%, Na3PO 4 10%, experimental seed treatment: 5% and 1.25% of component A and B, respectively. Seeds, at first disinfected from virus contamination by means of heat treatment (70oC) and the above listed compounds and concentrations, can be treated furthermore with carbendazim plus thiram plus metalaxyl at doses of 0.6, 1.35 and 1.75 g per kg of seeds, respectively, to prevent fungus infection.

keywords:cucumber, seeds, virus, CGMMV, thermal and chemical disinfection

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