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VCRB :: Contents of Volume 50/1999 :: Detailed information


EVALUATION OF TWO TECHNIQUES FOR SCREENING TOMATOES FOR RESISTANCE TO FUSARIUM CROWN AND ROOT ROT

Elżbieta U. KOZIK
Research Institute of Vegetable Crops Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland


Summary

Development of tomato cultivars resistant to Fusarium lycopersici f. sp. radicis-lycopersici (FORL) requires precision in disease incitement and in rating plants for response to infection. Commonly disease rating is done by visual scoring of plant symptoms under variable field or greenhouse conditions. Other methodologies have recently been developed to examine host/pathogen relationships and to assess resistance and susceptibility under more controlled conditions. In this study, an in-vitro method is compared with the traditional growth chamber method to determine FORL reactions of eight tomato accessions that differed in visual FORL symptom expression. The in vitro test provided the same order of genotype ranking on the basis of disease severity index (DSI) observed with the soil method. However, the in vitro method gave a greater resolution of resistance status among the genotypes. The accessions could be divided into two groups as resistant and susceptible. Four susceptible varieties had statistically greater pathogen infection than four resistant accessions. Both methods provided qualitatively similar results, however the in vitro method, by reducing environmental variances and being simpler and quicker, could be important in the development and evaluation of new resistant tomato cultivars if incorporated into breeding programs.

keywords:FORL, Fusarium lycopersici f. sp. radicis-lycopersici, resistance, tomato

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