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VCRB :: Contents of Volume 59/2003 :: Detailed information


THE GENETIC DIVERSITY AMONG CULTIVARS OF SCORZONERA (SCORZONERA HISPANICA L.)


Andrzej RAFALSKI1, Agnieszka DOLOTA2, Iwona WIŚNIEWSKA1, Barbara DĄBROWSKA2
1Plant Breeding and Acclimatization Institute, Radzików, 05-870 Błonie, Poland 2 Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warsaw, Poland


Summary

The genetic variability between 7 commercial varieties of scorzonera (Scorzonera hispanica L.) was estimated using PCR-based marker analysis. The polymorphism at the DNA level was evaluated using semi-specific primers targeting intron-exon sequences of plant genes. The results of amplification with the use of 22 primers confirm the versatility of proposed PCR technique, which has been used mainly for evaluation of genetic differences among accessions of cereals. The evaluation of diversity between varieties of scorzonera was based on analysis of 513 DNA fragments The genetic distances between varieties were relatively high, the coefficients of dissimilarity varied from 0.291 to 0.355. This high genetic distances evaluated on DNA level are in accordance with observed variability of morphological characters of genus Scorzonera and differences between varieties. The results of amplification also indicate, that using semi-specific primers is easy to identify some cultivar-specific DNA fragments.
The cluster analysis based on PCR data discriminate two groups of varieties the old and the newest ones. The results presented indicate that essential problems with cultivation of scorzonera can be solved and this vegetable may acquire greater economic importance.


keywords:Scorzonera hispanica, cultivars, genetic distance, semi-specific PCR

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