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VCRB :: Contents of Volume 58/2003 :: Detailed information


MOLECULAR EVIDENCE FOR THE PRESENCE OF dsRNA VIRUS 
IN MUSHROOMS (AGARICUS BISPORUS) SHOWING 'LA FRANCE' DISEASE SYMPTOMS


Jędrzej MASZKIEWICZ1, Tadeusz MALINOWSKI2
1 Research Institute of Vegetable Crops Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland 2 Research Institute of Pomology and Floriculture Pomologiczna 18, 96-100 Skierniewice, Poland


Summary

The presence of virus associated with 'La France' disease was confirmed in the mushroom sporophores collected in 2000, on the farm located in Central Poland. Two different types of isometric virus-like particles (diameter 25 and 34-35 nm) were observed in electron microscope. After electrophoretic separation of dsRNA isolated from suspected material revealed five characteristic bands. After RT-PCR amplification, a specific PCR product of expected size (about 480 bp) was obtained from samples of infected sporophores. In few cases, a positive RT-PCR reaction was also found for apparently healthy mushrooms, suggesting the high incidence of latent infections with LFD.


keywords:mushrooms, virus diseases, identification, dsRNA

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